0000092135 00000 n [6] Tap water is sufficiently basic to give a solution of bromocresol green its characteristic blue-green color.[7]. Yahoo is part of Verizon Media. 0000007046 00000 n 0000008355 00000 n 0000001561 00000 n It is used as a pH indicator and as a tracking dye for DNA agarose gel electrophoresis. xÚb```b``Éd`e`�Šbd@ A ÇGe†‹Ì � Lo–ï�Í€d”˜U’VMšuÁågz“šdævÙM’kûº²Ö9Q0. Q4. 0000045863 00000 n It is also an inhibitor of the prostaglandin E2 transport protein. %%EOF 0000000016 00000 n PubChem Substance ID 24847166. In clinical practise, it is commonly used as a diagnostic technique. Calculate [bromocresol green], in mol/L, in the stock solution (0.04% (m/v)). To enable Verizon Media and our partners to process your personal data select 'I agree', or select 'Manage settings' for more information and to manage your choices. NACRES NA.47 Bromocresol green (BCG) is a dye of the triphenylmethane family (triarylmethane dyes). When it turns blue, the pH is 5.52. *Please select more than one item to compare Empirical Formula (Hill Notation) C 21 H 14 Br 4 O 5 S . 0000092640 00000 n 76-60-8. It can be used in its free acid form (light brown solid), or as a sodium salt (dark green … 0000007988 00000 n BROMCRESOL GREEN. Compare Products: Select up to 4 products. The most common use of Bromocresol green is to measure serum albumin concentration within mammalian blood samples in possible cases of kidney failure and liver disease. 0000005499 00000 n EC Number 200-972-8. Molecular Weight 698.01 . 0000001687 00000 n 2471 35 Precise pH measurement Absorption spectra of bromocresol green at different stages of protonation. 0000009967 00000 n 0000003401 00000 n 0000007814 00000 n startxref In aqueous solution, bromocresol green will ionize to give the monoanionic form (yellow), that further deprotonates at higher pH to give the dianionic form (blue), which is stabilized by resonance: 3,3-Bis(3,5-dibromo-4-hydroxy-2-methylphenyl)-3H-benzo[c][1,2]oxathiole 1,1-dioxide Why can we determine the Ka of bromocresol green only semi-quantitatively? Find out more about how we use your information in our Privacy Policy and Cookie Policy. 2471 0 obj <> endobj 0000002106 00000 n In aqueous solution, bromocresol green will ionize to give the monoanionic form (yellow), that further deprotonates at higher pH to give the dianionic form (blue),[5] which is stabilized by resonance: The acid dissociation constant (pKa) of this reaction is 4.8. Bromocresol Green ACS reagent, Dye content 95 % CAS Number 76-60-8. Bromocresol green (3,3',5,5'-tetrabromo-m-cresolsulfonphthalein) is a dye of the triphenylmethane family (triarylmethane dyes), which is used as a pH indicator and as a tracking dye for DNA agarose gel electrophoresis. a) What relationship does the pKa bear to the Ka? An ethanol solution (0.04 wt%) of bromocresol green has been proposed for TLC staining and is suitable for visualisation of the compounds with functional groups whose pKa is below 5.0 (carboxylic acids, sulfonic acids, etc.). 0000015809 00000 n 0 0000007950 00000 n When it turns green, the pH is 3.88. Skin and eye contact should be avoided. %PDF-1.6 %âãÏÓ 0000108158 00000 n Bromocresol green. xref You can change your choices at any time by visiting Your Privacy Controls. These appear as yellow spots on a light or dark blue background; no heating is necessary. <]>> 0000011461 00000 n Bromocresol green may cause irritation. 0000005250 00000 n 0000000996 00000 n trailer Bromophenol blue solution can be used for the same purpose. 0000005734 00000 n Additional applications include use in sol-gel matrices,[8] the detection of ammonia,[9] and the measurement of albumin in human plasma and serum.[10]. Search results for bromocresol green at Sigma-Aldrich. 0000004995 00000 n 0000003938 00000 n We and our partners will store and/or access information on your device through the use of cookies and similar technologies, to display personalised ads and content, for ad and content measurement, audience insights and product development. It belongs to a class of dyes called sulfonephthaleins. In order to determine the pKA of bromcresol green, a series of measurements must be performed at different pHs. 0000001428 00000 n bromo cresol green. Q5. 0000006319 00000 n [4] It is used as a pH indicator in applications such as growth mediums for microorganisms and titrations. 2505 0 obj<>stream 0000005812 00000 n The compound is synthesized by bromination of cresol purple (m-cresolsulfonphthalein). The pKA is equal to the pH at which the concentration of the two forms are equal. 2,6-Dibromo-4-[7-(3,5-dibromo-4-hydroxy-2-methyl-phenyl)-9,9-dioxo-8-oxa-9λ6-thiabicyclo[4.3.0]nona-1,3,5-trien-7-yl]-3-methyl-phenol, InChI=1S/C21H14Br4O5S/c1-9-12(7-14(22)19(26)17(9)24)21(13-8-15(23)20(27)18(25)10(13)2)11-5-3-4-6-16(11)31(28,29)30-21/h3-8,26-27H,1-2H3, InChI=1/C21H14Br4O5S/c1-9-12(7-14(22)19(26)17(9)24)21(13-8-15(23)20(27)18(25)10(13)2)11-5-3-4-6-16(11)31(28,29)30-21/h3-8,26-27H,1-2H3, Brc1cc(c(c(Br)c1O)C)C3(OS(=O)(=O)c2ccccc23)c4cc(Br)c(O)c(Br)c4C, Except where otherwise noted, data are given for materials in their, http://chemicalland21.com/specialtychem/finechem/BROMOCRESOL%20GREEN.htm, "BCG (Bromocresol Green) Albumin Assay Kit", https://en.wikipedia.org/w/index.php?title=Bromocresol_green&oldid=961946517, Pages using collapsible list with both background and text-align in titlestyle, Articles containing unverified chemical infoboxes, Creative Commons Attribution-ShareAlike License, Soluble in benzene; very soluble in ethanol and diethyl ether, This page was last edited on 11 June 2020, at 07:31. The pKa of bromocresol green is given in the Merck Index. 0000092368 00000 n It can be used in its free acid form (light brown solid), or as a sodium salt (dark green solid). 0000083050 00000 n 0000004456 00000 n The acid and basic forms of this dye have an isosbestic point in their UV-Visible spectrum, around 515 nm, indicate that the two forms interconvert directly without forming any other substance. Buy Sigma Aldrich 114359-25G Bromocresol Green, ACS Reagent Grade, 25g Bottle 0000009231 00000 n 0000006062 00000 n 0000010771 00000 n 0000002660 00000 n When bromocresol green is added to a solution, the pH is 2.88 (the color is yellow). b) What is the literature value of the pKa for bromocresol green (Merck Index)? Beilstein/REAXYS Number 372527 . Information about your device and internet connection, including your IP address, Browsing and search activity while using Verizon Media websites and apps. MDL number MFCD00005874. 0000012364 00000 n 0000013138 00000 n

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